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Ellman’s reagent (5,5'-dithiobis (2-nitrobenzoic acid) [6, 7], O-phthalic aldehyde [8], 4-vinyl pyridine, 4-fluoro-7-sulfobenzofurazan ammonium salt, 4- (aminosulfonyl)-7-fluorobenzofurazan and N- (9-acridinyl) maleimide [9] are used as derivatizing agent