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, duodenal venous congestion lesions, perforated cecum, ischemic/reperfusion colitis, bile duct ligation-induced liver cirrhosis and portal hypertension, temporary portal triad occlusion (ischemia-reperfusion injury following the Pringle maneuver), and suprahepatic occlusion of the inferior caval vein (Budd-Chiari syndrome)]
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Digestion-based DNA methylation analysis Percentage DNA methylation was assessed at CpG sites across CpG islands in CYP2R1, CYP27A1, CYP27B1, CYP24A1, and VDR genes using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer