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The nonlinear plot in Figure 2 presents the calculation of mean ± SEM for the spans of 50 B 12 units (for values up to 1000 pg/mL), and then for 200 B 12 units wide sections (above 1000 pg/mL), and then the saturation curve was fitted ( Hb 0 = 0.4254, Hb M = 14.59, K = 5.076)